One drawback is the presences of new antigens due to possible heat denaturation of the albumin.
2.
The calcium-dipicolinic acid complex also functions to protect DNA from heat denaturation by inserting itself between the nucleobases, thereby increasing the stability of DNA.
3.
Because UDG does not react with dUTP, and is also inactivated by heat denaturation prior to the actual PCR, carry-over contamination of PCRs can be controlled effectively if the contaminants contain uracils in place of thymines.
4.
Heat denaturation of DNA, also called melting, causes the double helix structure to unwind to form single stranded DNA . When DNA in solution is heated above its melting temperature ( usually more than 80 �C ), the double-stranded DNA unwinds to form single-stranded DNA . The bases become unstacked and can thus absorb more light.
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